PENICILLINASE PLASMID DNA FROM Staphylococcus aureus

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Constitutive penicillinase formation in Staphylococcus aureus owing to a mutation unlinked to the penicillinase plasmid.

Previously described penicillinase-constitutive mutations in Staphylococcus aureus are caused by genetic lesions in a regulator gene (or genes) on the penicillinase plasmid in close linkage to the structural gene. This report describes a new class (R2(-)) of penicillinase-constitutive mutants of S. aureus unlinked to the plasmid. By transductional analysis, the penicillinase plasmids in these m...

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Regulation of penicillinase synthesis: a mutation in Staphylococcus aureus unlinked to the penicillinase plasmid that reduced penicillinase inducibility.

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Nature of the elimination of the penicillinase plasmid from Staphylococcus aureus by surface-active agents.

Growth of Stapylococcus aureus in various ionic surface-active agents resulted in loss of the ability to produce penicillinase, whereas growth in nonionic surface-active agents had no effect on penicillinase production. The curing effect of various alkyl sulfates was found to be dependent upon the chain length. Curing by surface-active agents could be inhibited by magnesium. Reciprocal transduc...

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Regulation of Penicillinase Synthesis : a Mutation in Staphylococcus aureus Unlinked to the Penicillinase Plasmid That Reduces Penicillinase Inducibility

A mutant of Staphylococcus aureus strain 655 was isolated that is restricted in penicillinase induction. Wild-type plasmids that bear penicillinase determinants could not be fully induced in this mutant, 655par-1; hence, the responsible mutation is not located on the plasmid. Mutant plasmid PI258 penI443, which produces penicillinase constitutively in wild-type cells, was fully constitutive for...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1969

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.63.4.1304